NGS测序基础01-文库构建（Library Preparation） - 知乎专栏
1）PE adapter添加; 利用碱基互补配对的原则，加上PE adapter。PE adpater中一部分是建库PCR富集时候需要用的引物序列，另一部分是测序时需要用的引物。 2）PCR 添加 index，P5，P7
高通量测序中的接头（adapter）到底是什么 - 简书
2018年10月1日 · 待测序的目标序列，位于两个adapter之间. 测序片段示意图. 测序片段包括几个部分：universal_adapter-insert-indexed_adapter。. 测序由5'端开始，最开始的几个碱基无法测得，第一个adapter在数据输出时去除，由于测序读长的限制，第二个adapter通常测不到。. 但是如果 …
Adapter Ligation technology | Flexibility for many study designs
Adapter ligation technology has long been known for high coverage uniformity, precise strand information, and reliable library preparation, even from degraded samples. Now it’s even better. A universal, methylated adapter design incorporates an index sequence at the initial ligation step.
Adapter Ligation technology | Flexibility for many study designs
Adapter ligation technology constructs NGS libraries for sequencing using an enzyme that connects specialized adapters to both ends of DNA fragments.
Library adaptors with integrated reference controls improve the ...
2022年10月28日 · Library adaptors are short oligonucleotides that are attached to RNA and DNA samples in preparation for next-generation sequencing (NGS). Adaptors can also include additional...
Ligation-based library preparation - Integrated DNA Technologies
Adapter ligation—A ligase enzyme covalently links the adapter to the DNA fragments, making a complete library molecule. Adapters serve multiple functions: they can attach the sequences to a flow cell or ensure sequencing platform-specific compatibility.
Targeted nanopore sequencing with Cas9-guided adapter ligation
2020年2月10日 · In the present study, we describe nanopore Cas9-targeted sequencing (nCATS), an enrichment strategy that uses targeted cleavage of chromosomal DNA with Cas9 to ligate adapters for nanopore...
Systematic evaluation and optimization of the experimental
2019年5月30日 · In this study, we have systematically evaluated and optimized the 5 key experimental steps of the experimental pipeline in rG4-seq, namely 3′ dephosphorylation, 3′-adapter ligation, excess 3 ...
DNA Library Preparation - Illumina
The other key technology used in our NGS library prep is adapter ligation, long known for consistent, high-quality data. Libraries are prepared by fragmenting a gDNA or cDNA sample and ligating specialized adapters to both fragment ends. These adapters contain the full complement of sequencing primer hybridization sites.
Preparation of DNA Sequencing Libraries for Illumina Systems—6 …
In library preparation, a stoichiometric excess of adapters relative to sample DNA is used to help drive the ligation reaction to completion. Ligation efficiency is critical for conversion of DNA fragments into sequenceable molecules and thus impacts …