Simplify bioreactor scale up and scale down - Cytiva
Bioreactor scale up and scale down are important but can be complex. Simplify with Cytiva’s web-based bioreactor scaling tool. View several case studies.
Bioreactor Scale-Up - SpringerLink
2019年7月16日 · In this chapter, main factors and issues surrounding bioreactor scale-up in different types of bioreactors are discussed. Furthermore, we will review mass and heat transfer phenomena with an emphasis on their role in scale-up.
A practical approach in bioreactor scale-up and process ... - PubMed
Here we describe a practical approach in bioreactor scale-up and process transfer, where bioreactor information may be limited. We evaluated the sparger kLa and kLaCO2 (CO 2 volumetric mass transfer coefficient) from a range of bioreactor scales (3 …
Exploring Principles of Bioreactor Scale-Up - bioprocessintl.com
2024年2月7日 · A biologic manufacturing process typically involves scaling up of production from miniaturized, high-throughput bioreactors (15–250 mL) to bench-scale glass or single-use reactors (1–10 L) and then pilot- and production-scale bioreactors (200–5,000 L or larger).
What to consider when scaling-up your bioreactor? - Cytiva
2020年10月16日 · When scaling-up your bioreactor, it is important to consider tech transfer and to define operating conditions, such as agitation rate and gas sparge rate.
Bioreactor scaling tool | Cytiva
Bioreactor Scaler is a software platform that predicts scaling in silico to help you define key process parameters, enabling seamless tech transfer. Both Scaler (free) and Scaler Pro deliver key data-driven insights that help biomanufacturers scale right the first time with less process waste and reduced overall risk.
The traditional method for scale-up of a fermentation process involves determining the reactor geometry, impeller speed, and aeration rate of the large-scale bioreactor on the basis of the experimental results of the lab-scale bioreactor.
Simplifying the Scaling Process Between Bioreactors - Sartorius
2021年11月4日 · Small bioreactors can sometimes be challenging to scale optimally, but by using tools such as bioreactors designed for geometric scale-up, process insights software, and tools for data analytics, you can save time and de-risk the transfer between scales.
extensive physical and CFD-based characterization of bioreactor mass transfer, shear regime, power input, mixing time, and tip speed. The tool guides the user to scalable and suitable operating
Digitally enabled approaches for the scale up of mammalian cell bioreactors
2022年9月1日 · Several factors contribute to the difficulty of scaling. These include the complexity of biological systems and the significant cost, including media and reagents, associated with transferring and testing processes within large-scale bioreactors (Carpio, 2020).
Bioreactor scale-up is a critical step in the production of biologics such as therapeutic proteins, monoclonal antibodies (mAbs), and vaccines. Scaling up can be complex; it requires choosing the optimal media formulations and securing reliable supply chains, while maintaining product quality and remaining cost-efective.
Strategies and engineering aspects on the scale-up of bioreactors …
This review presents the most employed bioreactors’ scale-up strategies. In addition, the scale-up of other bioreactors models, such as pneumatic and solid-state fermentation bioreactor and even photobioreactors, will also be described with some examples.
Bioreactor scale-up and oxygen transfer rate in microbial ... - PubMed
Theoretical predictions of both the volumetric mass transfer coefficient and the enhancement factor that have been recently proposed are described; finally, different criteria for bioreactor scale-up are considered in the light of the influence of OTR and OUR affecting the dissolved oxygen concentration in real bioprocess. Publication types.
Bioreactor design and scale-up with CFD modeling | Cytiva
Computational fluid dynamics (CFD) modeling in bioreactor design can reduce wet testing by simulating bioreactor geometry, fluid flow, and design and positioning of components.
Bioreactor scale-up and oxygen transfer rate in microbial ...
2009年3月1日 · Oxygen transfer is often the rate-limiting step in the aerobic bioprocess due to the low solubility of oxygen in the medium. The correct measurement and/or prediction of the volumetric mass transfer coefficient, (k L a), is a crucial …
Bioreactor scale-up and oxygen transfer rate in microbial processes…
2009年3月1日 · The correct measurement and/or prediction of the volumetric mass transfer coefficient, (kLa), is a crucial step in the design, operation and scale-up of bioreactors.
In the laboratory scale, up to volumes of approximately 15 L, the vessel is mostly made of glass, as glass is transparent, easy to clean, inert, and steam-sterilizable. Examples for bioreactors made of glass are shown in Figs. 5.2–5.5.
(27m) Bioreactors Scale-up and Optimization from Lab to ... - AIChE
A manufacturing scale bioreactor should have proper mixing and mass transfer but low shear to avoid damaging the microorganisms and biological cells. Optimized gas-liquid reactor results in time savings and increased profitability. However, design, scale-up, and optimization of these multiscale multiphase reactors are all still challenges in ...
7 factors that affect oxygen transfer to cells in bioreactors
The conventional scale‑up of bioprocesses is based on physicochemical and geometric similarity. kLa is kept constant for this scenario. The OTR should remain constant for a bioreactor platform with geometric similarity (such as Xcellerex™ bioreactors).
Bioreactor Design and Scale-Up | SpringerLink
This chapter comprises engineering aspects of bioreactor systems (design, operation, scale-up) developed or adapted for cultivation of mammalian cells, such as bioreactors for suspension culture (stirred-tank reactors, bubble columns, and air-lift reactors), fixed bed and fluidized bed reactors, hollow fiber and membrane reactors, and, finally ...
Bioreactor Scale‐Up - Xu - - Major Reference Works - Wiley …
2018年12月20日 · Proper bioreactor scale‐up is needed to ensure productivity and product quality. Here, common approaches and challenges in industrial mammalian cell culture scale‐up are covered.
Identifying and optimizing critical process parameters for large-scale ...
5 天之前 · Recent research indicates that a bioreactor scale-up approach can effectively expand and differentiate iPSCs while reducing the need for manual interventions in static cultures [11,12,13,14,15,16,17]. Already established differentiation protocols were conducted side by side in adherent and suspension environments. The results indicated that the ...