Threonine 68 of Chk2 Is Phosphorylated at Sites of DNA Strand …
2001年12月21日 · To investigate the phosphorylation and activation of Chk2, we raised polyclonal anti-phospho-Thr 68 antibodies against a Chk2 peptide containing T68P (CETVST(PO 4)QELYS). The specificity of these affinity-purified antibodies was examined by Western blot analysis.
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb - Cell Signaling …
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb detects endogenous levels of Chk2 only when phosphorylated at Thr68. Non-specific staining of mitotic cells was observed by immunohistochemistry.
Here we show that the Thr68-phosphorylated form of Chk2 (Chk2T68P) forms dis-tinct nuclear foci in response to ionizing radiation. Only this activated form localizes at sites of DNA strand breaks. The kinase activity of Chk2 and the number of Chk2 foci formed depend on the severity of DNA damage and gradually decrease with time.
Phospho-CHEK2 (Thr68) antibody (29012-1-AP) | Proteintech
Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68.
Chk2磷酸化抗体pT68(ab3501)|Abcam中文官网
Western blot - Anti-Chk2 (phospho T68) antibody (ab3501) The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.
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Threonine 68 of Chk2 Is Phosphorylated at Sites of DNA Strand …
2002年1月1日 · Chk2−/− embryonic stem cells failed to maintain γ-irradiation–induced arrest in the G2 phase of the cell cycle. Chk2−/−thymocytes were resistant to DNA damage–induced apoptosis.
Phosphorylation of Chk2 (P T68 -Chk2) persists after CPT
Screen —To further evaluate the importance of MRN for Chk2 activation by CPT, we examined Chk2 phosphorylation (on Thr-68) in each of the seven colon carcinoma cell lines from the NCI...
重组Chk2磷酸化抗体pT68[Chk2T68-D12](ab278548)|Abcam
All lanes : Anti-Chk2 (phospho T68) antibody [Chk2T68-D12] (ab278548) at 1 µg/ml. Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) untreated, whole cell lysate. Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) treated with UV, whole cell lysate. Predicted band size: 61 kDa.
CHK2 kinase: cancer susceptibility and cancer therapy - Nature
CHK2 is a versatile and multifunctional kinase that regulates the cell's response to DNA damage by phosphorylating a number of distinct cellular substrates. CHK2 can prevent tumour...
phosphorylation of Chk1 Ser345 (Chk1-S345p) and Chk2 Thr68 (Chk2-T68p) in MDA-MB-231 and A549 cells following exposure to WYC02 or WYC0209 at indicated doses and times. Cells treated with 0.1 mM H2O2 and 10 J/m 2 UV for 1 h served as positive control. B, cytotoxicity test for WYC02, WYC0209, and cisplatin on A549 and MDA-MB-231 cells. Data are ...