Cryoprotectant - an overview | ScienceDirect Topics
Cryoprotectants are one of the important factors in cryopreservation, and they are currently an active research area. The ideal cryoprotectant should possess high water solubility, permeability, low toxicity, non-reactivity, and no precipitation at high concentrations.
Chemical approaches to cryopreservation - Nature Reviews …
2022年7月18日 · A major challenge with this method is cryoprotectant toxicity and the need for rapid but stepwise removal of the CPA to avoid post-thaw toxicity and osmotic damage.
Cryoprotectants: A review of the actions and applications of ...
2017年6月1日 · The definition of a cryoprotectant is any solute which, when added to cells in their medium, allows higher post-thaw recoveries than if it were not present [143]. However, before discussing the nature of CPAs and these applied definitions, it is worth considering the challenges that are imposed by cooling to ultra-low temperatures — i.e. the ...
dissolved molecules prevent water from freezing completely. With cryoprotectant, the percentage of cryoprotectant present in solution increases as ice grows. At any given tem-perature, ice growth stops when the cryoprotectant becomes concentrated enough to make the melting point equal to the surrounding temperature. Eventually the cryoprotec-
The need for novel cryoprotectants and cryopreservation protocols ...
2021年1月1日 · Carbohydrates offer interesting insights into the mechanisms of cryoprotectant activity. One study found that disaccharides were twice as effective at preventing ice recrystallization as monosaccharides but at high concentrations the disaccharides were more toxic to the cell lines tested [ 51 ].
Chemical approaches to cryopreservation - PMC
For example, a new cryoprotectant that cannot be washed/removed before transfusion to a patient must meet strict regulatory and safety parameters. In contrast, a cryoprotectant used upstream, or in basic research, can be more readily applied and used.
Cryopreservation: A Review Article - PMC
Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant. Liu J, Tanrikut C, Wright DL, Lee GY, Toner M, Biggers JD, Toth TL. Cryobiology. 2016;73:162–167. doi: 10.1016/j.cryobiol.2016.08.004.
How cryoprotectants work: hydrogen-bonding in low-temperature …
2022年8月8日 · These studies revealed the precise mechanism of how DMSO acts as a cryoprotectant by disrupting the tetrahedral H-bond structure, by disordering water molecules within its first hydration shell.
Ice Inhibition for Cryopreservation: Materials, Strategies, and ...
2021年2月1日 · The recent development of chemical ice-inhibition molecules, including cryoprotectant, antifreeze protein, synthetic polymer, nanomaterial, and hydrogel, and their applications in cryopreservation are summarized.
Coping with the cold: unveiling cryoprotectants, molecular …
The intricate relationship between these cryoprotectant molecules and cellular processes enhances the plant’s resilience to cold stress and bolsters survival in freezing environments.
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