Extraction of DNA from soil - ScienceDirect
2003年10月1日 · Two strategies have been developed to improve DNA recovery in terms of yield, purity and unbiased representation of the microbial diversity. The first approach consists of the …
A Rapid and Economical Method for Efficient DNA Extraction …
2015年7月13日 · A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for environmental microbiology. The present work describes an improved method of …
Soil Microbiome Study Based on DNA Extraction: A Review - MDPI
2022年12月8日 · To overcome the challenges, advanced and more comprehensive molecular tools based on the analysis of nucleic acids (DNA and RNA) extracted directly from the soil are …
Extraction and purification of microbial DNA from soil and …
2001年9月1日 · Extracted DNA provides a less biased view of soil microbial communities than do culture-based microbiological methods. However, these extraction methods all suffer from …
An Improved Method for High Quality Metagenomics DNA Extraction from ...
2016年5月31日 · In this study, we have developed an improved method for extraction of community DNA from different environmental and human origin samples. We introduced a …
Assessment of five soil DNA extraction methods and a rapid …
2014年2月1日 · We developed a simple and rapid alkali lysis based DNA extraction from soil. The laboratory-developed method could remove 90% of humic substances. The laboratory …
Methods for microbial DNA extraction from soil for PCR …
DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and …
Successive DNA extractions improve characterization of soil …
2017年2月1日 · Often, studies describing and comparing soil microbial communities are based on a single DNA extraction, which may not lead to a representative recovery of DNA from all …
The metagenomics of soil | Nature Reviews Microbiology
Soil metagenomics is a cultivation-independent molecular approach to explore and exploit the enormous diversity of soil microbial communities. This technology comprises...
High-Throughput Isolation of Nucleic Acids from Soil - MDPI
2019年12月28日 · DNA extraction from the soil is a key step for these approaches: it is a challenge for researchers as it is still both expensive and time-consuming when large surveys …