Fluorescence spectroscopy - Wikipedia
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample.
Spectrofluorometer - Wikipedia
A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.
General. All fluorescence instruments contain three basic items: a source of light, a sample holder and a detector. In addition, to be of analytical use, the wavelength of incident radiation needs to be selectable and the detector signal capable of precise manipulation and presentation (Figure 5).
Spectrofluorometry - an overview | ScienceDirect Topics
Spectrofluorimetry is a highly sensitive analytical method for the detection and determination of fluorescent compounds at ng or lower level. It has extensively been applied to the analysis of multicomponent systems and some applications in the field of pharmaceutical and clinical analysis are presented as follows.
Principles and Theory of Fluorescence Spectroscopy - HORIBA
Principles and Theory of Fluorescence Spectroscopy. Fluorescence is a type of luminescence caused by photons exciting a molecule, raising it to an electronic excited state. It’s brought about by absorption of photons in the singlet ground state promoted to a singlet-excited state.
Spectrofluorimetry - SpringerLink
Jan 1, 2011 · In the introduction of this chapter we pointed out that a crucial advantage of spectrofluorimetry is the possibility of obtaining a linear response over a wide concentration range.
What is Fluorescence, What is a Spectrofluorometer ...
Fluorescence is the property of some atoms and molecules to absorb light at a particular wavelength. A microplate reader with fluorescence intensity (FI) detection uses a light to excite a fluorescent molecule.