Five-prime cap - Wikipedia
In molecular biology, the five-prime cap (5′ cap) is a specially altered nucleotide on the 5′ end of some primary transcripts such as precursor messenger RNA. This process, known as mRNA capping, is highly regulated and vital in the creation of stable and mature messenger RNA able to undergo translation during protein synthesis.
5-prime capping of mRNA - Takara Bio
The cap structure at the 5′ end of mRNA is critical for the efficient translation, stabilization, and transportation of mRNAs in eukaryotes. RNA capping is an essential part of the workflow when mRNA synthesized in vitro (in vitro-transcribed or IVT mRNA) for …
mRNA capping: biological functions and applications
2016年6月17日 · In addition to its essential role of cap-dependent initiation of protein synthesis, the mRNA cap also functions as a protective group from 5′ to 3′ exonuclease cleavage and a unique identifier for recruiting protein factors for pre …
Capping enzyme - Wikipedia
A capping enzyme (CE) is an enzyme that catalyzes the attachment of the 5' cap to messenger RNA molecules that are in the process of being synthesized in the cell nucleus during the first stages of gene expression. The addition of the cap occurs co-transcriptionally, after the growing RNA molecule contains as little as 25 nucleotides.
mRNA capping: biological functions and applications - PubMed
2016年9月19日 · In this review, we will summarize the current knowledge of the biological roles of mRNA caps in eukaryotic cells. We will also discuss different means that viruses and their host cells use to cap their RNA and the application of these capping machineries to synthesize functional mRNA.
Mechanisms of transcription and RNA processing - Nature
2024年5月14日 · Structures of complex mRNA processing assemblies have also become possible, revealing insights into capping, splicing and 3′ end processing. In particular, our understanding of splicing...
Conventional and unconventional mechanisms for capping viral mRNA …
2011年12月5日 · In the eukaryotic cell, capping of mRNA 5′ ends is an essential structural modification that allows efficient mRNA translation, directs pre-mRNA splicing and mRNA export from the nucleus,...
mRNA cap regulation in mammalian cell function and fate - PMC
The mRNA cap is a highly methylated modification of the 5′ end of RNA pol II-transcribed RNA. It protects RNA from degradation, recruits complexes involved in RNA processing, export and translation initiation, and marks cellular mRNA as “self” to …
Synthetic mRNA capping - PMC
In this review article, we present different synthetic routes to 5′-capped mRNAs based on enzymatic, chemical or chemo-enzymatic methods. We will point out the difficulties and limitations of the different strategies and – if available – will show ways to circumvent them.
mRNA cap regulation in mammalian cell function and fate
The mRNA cap is a highly methylated modification of the 5' end of RNA pol II-transcribed RNA. It protects RNA from degradation, recruits complexes involved in RNA processing, export and translation initiation, and marks cellular mRNA as "self" to …