Ultraviolet Detectors: Perspectives, Principles, and Practices
2019年10月1日 · The principle for UV detection is Beer's law, also called the Beer-Lambert law, where. Absorbance (A) = molar absorptivity (ε) × pathlength (b) × concentration (c) …
Chromatography Online
RAFA 2024 Highlights: Cutting-Edge Chromatography Techniques for Food Safety and Food Analysis. An illuminating session focusing on progress in analytical techniques used in food …
The Flame Ionization Detector - Chromatography Online
2005年12月1日 · The Flame Ionization Detector. Flame ionization detection (FID) is the most commonly used gas chromatography (GC) detection method. Flame ionization detectors …
What Chromatograms Can Tell Us About Our Analytes
2021年1月1日 · A chromatogram can provide a wealth of information about an individual analyte in a sample, about the sample itself, and about how well the instrument is performing. …
Troubleshooting Basics, Part IV: Peak Shape Problems
2012年7月1日 · A buffer concentration of 5–10 mM usually is adequate to buffer the mobile phase, column, and injection solvent in reversed-phase separations. If buffer concentration …
The Essence of Modern HPLC: Advantages, Limitations, …
2013年6月1日 · Retention Selectivity Efficiency Conventional wisdom leads us to the following "rules of thumb" for isocratic analysis. First, k or retention factor is the ratio of retention times …
LCGC North America - Chromatography Online
LCGC North America. ... Practical advice, tutorials, and peer-reviewed research on liquid chromatography (HPLC), gas chromatography (GC), mass spectrometry (MS) and related …
Back to Basics: The Role of pH in Retention and Selectivity
2017年1月1日 · Peak 3 (orange) has a similar p Ka (2.75), so very similar behavior is seen for peaks 3 and 4, with stable retention above pH 4.0. Although the retention times of these two …
Mobile-Phase Degassing: What, Why, and How - Chromatography …
2014年7月1日 · If mobile-phase outgassing occurs within the LC system, the most common problem areas are the pumps and detector. At the extreme, air in the pump will cause the …
Chromatography Fundamentals, Part III: Retention Parameters of …
2018年7月1日 · where d 0 is the migration distance of the solvent front measured from the bottom of the plate in contact with developing solvent, and d r is the migration distance of the solute …