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Articles | BMC Developmental Biology - BioMed Central
2021年11月4日 · Dexamethasone priming enhances stemness and immunomodulatory property of tissue-specific human mesenchymal stem cells. Human Mesenchymal Stem Cells (hMSCs) represent a promising cell source for cell-based therapy in autoimmune diseases and other degenerative disorders due to their immunosuppressive, anti-inflammatory and regen...
Long-term expansion, genomic stability and in vivo safety of adult ...
2020年2月26日 · Culture of hPOs with a chemically defined, biomimetic, ECM. The ability of hPOs to undergo expansion and maintain genomic stability both in vitro and in vivo makes them a promising platform for use in basic cellular studies of human pancreas biology, disease modelling and as a cellular therapy.
Notch signaling in mouse blastocyst development and hatching
2020年6月2日 · Effect of pharmacological Notch signaling inhibition and activation on gene transcription in 4.0 dpc mouse expanded blastocysts. Mouse 2.5 dpc embryos were in vitro cultured in the presence of a Notch inhibitor (DAPT) or of Notch ligands Jagged1 and Jagged2, for 36 h, until 4.0 dpc.
A sensitive and bright single-cell resolution live imaging reporter …
2010年12月21日 · Understanding the dynamic cellular behaviors and underlying molecular mechanisms that drive morphogenesis is an ongoing challenge in biology. Live imaging provides the necessary methodology to unravel the synergistic and stereotypical cell and molecular events that shape the embryo.
In situ hybridization protocol for enhanced detection of gene ...
2013年3月12日 · The freshwater planarian Schmidtea mediterranea has emerged as a powerful model for studies of regenerative, stem cell, and germ cell biology. Whole-mount in situ hybridization (WISH) and whole-mount fluorescent in situ hybridization (FISH) are critical methods for determining gene expression patterns in planarians.
Proteomics of early zebrafish embryos - BMC Developmental Biology
2006年1月13日 · While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed. As a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos.
The developmental basis of mesenchymal stem/stromal cells …
2015年11月20日 · From the perspectives of both cell lineage specification and mesoderm germ layer patterning, developmental ontogeny of the three main mesoderm cell types of concern to the MSC biology, the adipocytes, osteoblasts and chondrocytes, will be discussed in detail.
Cre reporter strains produced by targeted insertion of
2001年3月27日 · Several Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, following Cre-mediated excision of a loxP -flanked transcriptional "stop" sequence. These mice are useful for cell lineage tracing experiments as well as for monitoring the expression ...
Drosophila mitoferrinis essential for male fertility: evidence for a ...
2010年6月21日 · In this work, we show that mutations in the Drosophila mitoferrin gene result in male sterility caused by developmental defects. From the sensitivity of the hypomorph mutants to low food iron levels we conclude that mitochondrial iron is essential for spermatogenesis.