Structural Basis of Bifunctional CTP/dCTP Synthase
网页Cryo-EM resolves the structure of Drosophila CTP/dCTP synthase (CTPS) with dNTPs at 2.7 Å. • dATP acts similarly to ATP as a substrate in CTPS. • dUTP is a less efficient substrate than UTP for CTPS. • dGTP is a weaker regulator than GTP for CTPS. • CTP and dCTP induce CTPS filamentation and inhibition. • Model explains CTPS dual ...
Structural basis of bifunctional CTP/dCTP synthase - PubMed
网页The final step in the de novo synthesis of cytidine 5'-triphosphate (CTP) is catalyzed by CTP synthase (CTPS), which can form cytoophidia in all three domains of life. Recently, we have discovered that CTPS binds to ribonucleotides (NTPs) to form filaments, and have successfully solved the structures of Drosophila melanogaster CTPS bound with ...
Quantitation of deoxynucleoside triphosphates by click reactions …
网页2020年1月17日 · For dTTP quantification, the use of 1,000 pmol of C8-alkyne-dCTP for labeling may lead to potential competition between C8-alkyne-dCTP and dCTP during DNA polymerization, as described by...
Biosynthesis of deoxynucleoside triphosphates, dCTP and dTTP: reaction …
网页2005年2月1日 · The enzyme reaction mechanism and kinetics for biosyntheses of deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) from the corresponding deoxycytidine diphosphate (dCDP) and deoxythymidine diphosphate (dTDP) catalyzed by pyruvate kinase were studied. The kinetic model for the two synthetic reactions was found to follow the ...
Structural basis of bifunctional CTP/dCTP synthase - bioRxiv
网页2023年2月20日 · We have recently found that CTPS combines with NTPs to form filaments, and solved the structures of CTPS bound with NTPs. Previous biochemical studies have shown that CTPS can also serve as dCTPS, i.e. CTPS/dCTPS can not only bind UTP, ATP and GTP to generate CTP, but also bind deoxynucleotides to generate dCTP.
Differential control of dNTP biosynthesis and genome integrity ...
网页2017年7月20日 · Here we determined the relationship between dTTP biosynthesis (dTTP/dCTP balance) and the prevention of DNA uracilation in a mycobacterial model that encodes both the Dut and Dcd:dut enzymes, and...
Characterization of filament-forming CTP synthases from …
网页The reaction is exclusively catalyzed by an enzyme designated as CTP synthase (CTPS) finalizing the pyrimidine de novo synthesis pathway. Newly formed CTP (dCTP) molecules represent building blocks for nucleic acids, and thus are essential for gene transcription and for nucleic acid synthesis during cell division.
Structures of dCTP Deaminase from - Journal of Biological Chemistry
网页dCTP deaminase (EC 3.5.4.13) catalyzes the deamination of dCTP forming dUTP that via dUTPase is the main pathway providing substrate for thymidylate synthase in Escherichia coli and Salmonella typhimurium. dCTP deaminase is unique among nucleoside and nucleotide deaminases as it functions without aid from a …
Structural basis of bifunctional CTP/dCTP synthase - ResearchGate
网页2023年2月20日 · Previous biochemical studies have shown that CTPS can also serve as dCTPS, i.e. CTPS/dCTPS can not only bind UTP, ATP and GTP to generate CTP, but also bind deoxynucleotides to generate dCTP....
Enzymatic Synthesis of Nucleoside Triphosphates and …
网页2019年11月19日 · By using intact recombinant bacteria cells as a catalyst coupled with an ACKase-catalyzed adenosine-5′-triphosphate (ATP) regeneration system, nucleoside triphosphates (NTPs) and deoxynucleoside triphosphates (dNTPs) could be …
跳至内容