Mitotic Count and the Field of View Area: Time to Standardize
2015年12月28日 · The diameter in a field of view (FOV) with a light microscope is calculated by: FN (mm on ocular)/objective magnification = mm diameter of one field (Table 1). The limiting factor that determines the diameter width in the FOV …
Counting mitoses: SI(ze) matters! | Modern Pathology - Nature
2021年6月2日 · Those published prior to 2000 are likely to have used microscopes with small fields of view, as field diameters/areas have increased with the use of improved lens technology over recent...
As review of digital images in place of microscopic sections has become commonplace, establishment of a standard area in place of ‘‘high-power field enumeration’’ has become even more imperative. The field of view of digital images depends not only on the magnification but also on the size of the view-ing monitor.
Rapid 3D imaging at cellular resolution for digital ... - Nature
2024年10月1日 · Here, we introduce a new parallelized microscope for scanning thick specimens across extremely wide fields-of-view (54 × 72 mm 2) at 1.2 and 0.6 μm resolutions, accompanied by machine learning...
(Re) Defining the High-Power Field for Digital Pathology - PMC
The microscope high-power field (HPF) is the cornerstone for histopathology diagnostic evaluation such as the quantification of mitotic figures, lymphocytes, and tumor grading. With traditional light microscopy, HPFs are typically evaluated by quantifying histologic events in 10 fields of view at × 400 magnification.
Current applications and challenges of digital pathology in ...
2022年6月1日 · The increased demand for rapid on-site evaluation (ROSE), primary diagnosis, education, and biomarker quantification can be met by the use of telecytology, whole slide imaging (WSI), and digital image analysis solutions. In this review, we will focus on currently available clinical applications as well as challenges in digital cytopathology.
Comparison of glass slides and various digital‐slide modalities for ...
2017年5月30日 · When the cytotechnologist at the microscope moved a glass slide on the microscope stage, the Panoptiq software digitally captured the field of view and stitched it to prior fields of view in real time; this created a single panoramic (mosaic) image (in the SVS file format).
Evaluation and optimization for liquid-based preparation cytology …
2011年10月19日 · Today′s non-WSI, FDA approved, automated LBP cytology screeners utilize 3 planes during their process, determine the best focused field of view out of the three, and then use it for analysis.
“SMART” cytology: The next generation cytology for
2023年3月1日 · It uses a proprietary algorithm to identify the 22 most concerning fields of view (FOVs) on a ThinPrep slide based on image analysis morphometry. 2 Both the FocalPoint GS Imaging System and ThinPrep Imaging System have been commercially successful and widely adopted by cytology laboratories into their clinical workflow. 2 They addressed the ...
Increasing a microscope's effective field of view via overlapped ...
2022年1月17日 · This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis. Automatic detection, classification and counting of various morphological features of interest is now a crucial compone ….