Okazaki fragments - Wikipedia
网页Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication. [1]
Okazaki Fragments- Definition, Formation, Significances
网页2023年8月3日 · Okazaki fragments are short DNA nucleotide sequences with an RNA primer at the 5' end that are synthesized discontinuously and later joined by the enzyme DNA ligase to form the lagging strand during DNA replication.
High-fidelity DNA ligation enforces accurate Okazaki fragment ...
网页2021年1月20日 · DNA ligase 1 (LIG1, Cdc9 in yeast) finalizes eukaryotic nuclear DNA replication by sealing Okazaki fragments using DNA end-joining reactions that strongly discriminate against...
Okazaki fragment maturation: DNA flap dynamics for cell ... - Cell …
网页2022年7月22日 · Okazaki fragment maturation (OFM), the most frequently occurring DNA metabolic process, is efficient, faithful, and highly regulated, and it is crucial for maintaining genome integrity and cell survival. Unligated Okazaki fragments are a major source of DNA single-strand breaks in eukaryotic cells.
Genome-wide measurement of DNA replication fork …
网页2023年1月18日 · To reveal the location of replication initiation and termination in the human genome, we developed Okazaki fragment sequencing (OK-seq), a quantitative approach based on the isolation and...
Monitoring genome-wide replication fork directionality by Okazaki ...
网页2021年1月13日 · Here we describe a detailed protocol for isolating and sequencing Okazaki fragments from asynchronously growing mammalian cells, termed Okazaki fragment sequencing (Ok-seq), for the purpose...
Okazaki fragment maturation: DNA flap dynamics for cell …
网页Sequential post-translational modifications of FEN1 mediate its dynamic interactions with PCNA and timely exchange of Okazaki fragment enzymes. During lagging strand DNA replication in S phase, FEN1 is symmetrically methylated at arginine residues, primarily R192 (R192me2s FEN1).
DNA replication: Partners in the Okazaki two-step - Cell Press
网页2001年10月16日 · The correct processing of Okazaki fragments during lagging-strand DNA replication has a vital role in maintaining genome integrity. Recent findings suggest that, in eukaryotes, the processing of Okazaki fragments occurs by a two-step mechanism governed by the single-stranded DNA binding factor RPA.
DNA Replication Mechanisms - Molecular Biology of the Cell
网页On the lagging strand, the DNA replication machine leaves behind a series of unsealed Okazaki fragments, which still contain the RNA that primed their synthesis at their 5′ ends. This RNA is removed and the resulting gap is filled in by DNA repair enzymes that operate behind the replication fork (see Figure 5-13 ).
Okazaki Fragment - an overview | ScienceDirect Topics
网页An Okazaki fragment is a transient piece of DNA involved in lagging strand synthesis during DNA replication. It plays a role in stabilizing repeat sequences to prevent slippage and expansion, as observed in studies on yeast mutants.