Fluorophore - Wikipedia
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds .
荧光光谱-荧光基团选择指南-赛默飞 | Thermo Fisher Scientific - CN
Alexa Fluor 染料是一款深受信赖的经典荧光染料。 Alexa Fluor 染料荧光亮度高、超多光谱性质各异的选择，适合您多样的实验。 Brilliant Ultra Violet (BUV) 和 Brilliant Violet (BV) 染料使用聚 …
發色團 - 维基百科，自由的百科全书
助色基團 （英语：auxochrome） 是一群形成特定 官能基 的原子，這群原子會 與發色團結合並且改變發色團吸收的光波長或吸收的光強度。 羟基， 烷氧基， 氨基， 巯基 等基团与共轭体系相连，其孤对电子能与共轭体系的π电子共轭，使化合物紫外吸收光谱峰值向长波方向位移，并使颜色加深，这种效应称为助色效应，这些基团就称为助色基。 [5] 加酸顯色反應 是當 pH值 改變時，物質的顏色也會跟著改變，也是 酸鹼指示劑 的特性。 環境的酸鹼值在某範圍改變時，酸鹼指示 …
Fluorophore - an overview | ScienceDirect Topics
A fluorophore is a fluorescent chemical compound that can absorb photons in the “ground state,” leading to fluorescence emission and vibration relaxation in the “excited state.” The transition from ground state to excited state occurs very rapidly (over femtoseconds).
Fluorophore Selection Guide | Thermo Fisher Scientific - US
Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.
Caveat fluorophore: an insiders’ guide to small-molecule fluorescent ...
2021年12月23日 · Here, we review classic and modern small-molecule fluorophores across the electromagnetic spectrum and highlight the advantages and caveats of this type of label. Organic chemistry began with...
What are fluorophores? - Abcam
Fluorophores or fluorochromes are photoreactive chemicals that absorb and emit energy in a predictable fashion. They re-emit light when excited, making them useful tags for identifying and characterizing cells or molecules in a mixture, visualizing proteins, or …
Fluorescence Fundamentals | Thermo Fisher Scientific - US
Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes (Figure 1). A fluorescent probe is a fluorophore designed to respond to a specific stimulus or to localize within a specific region of a biological specimen.
10.3.1: Intrinsic and Extrinsic Fluorophores
2022年8月10日 · An intrinsic fluorophore is a ion, molecule or macromolecule that fluoresces strongly in it native form while an extrinsic fluorophore is a species that has been made to fluoresce strongly through reaction with a fluorometric reagent.
Photochemical Mechanisms of Fluorophores Employed in …
We evaluate the chemistry and photochemical mechanisms of fluorescent probes employed in SMLM. This Review provides guidance on the identification and adoption of fluorescent probes in single molecule localization microscopy to inspire the design of next-generation fluorescent probes amenable to single-molecule imaging.
- 某些结果已被删除