正负选择系统 - 百度百科
胸苷激酶蛋白（tk）可使无毒的丙氧鸟苷（ganc，也称为鸟嘌呤）转变为毒性核苷酸，而杀死细胞，因而可用丙氧鸟苷筛选排除随机整合的细胞株。故同源重组时，g418和 ganc都有抗性，随 …
正负筛选（neo正向+HSV-tk负向）原理 - CSDN博客
Jun 17, 2021 · 在同源重组时，tk基因将被切除而丢失，相反在随机整合时，所有的序列均保留（包括tk）。胸苷激酶蛋白（TK）可使无毒的丙氧鸟苷（GANC，也称为鸟嘌呤）转变为毒性核苷 …
[9] Use of fusions to thymidine kinase - ScienceDirect
Jan 1, 2000 · Full length HSV-tk can be used, complete from the first codon to the last. No amino acid linker (spacer) between HSV-tk and the second part of the protein seems to be necessary …
正负筛选法 - 百度百科
构建一种特殊的 载体，该载体含有一段与靶基因同源的序列，在这段序列的某一外显子中插入Neor基因作为正选择标记；在同循序列之外的3’末端，或3’，5’两个末端接上 单纯疱疹病毒 的 …
Introduction of the Negative Selection Marker into Replacement …
Sep 1, 1996 · In this report, we present an alternative procedure to introduce any negative selection marker at the ends of a replacement vector using a simple ligation reaction, thereby …
New positive/negative selectable markers for mammalian cells on …
They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by …
A new set of positive/negative selectable markers for …
Sep 18, 1998 · In the cases of Tk-BSD and Tk-bsr, the more effective the positive selection, the more GANC was required to kill all the cells. In conclusion, these five new markers function in …
The thymidine kinase/ganciclovir-mediated "suicide" effect is …
Jun 12, 1995 · The herpes simplex virus thymidine kinase (HSV-TK) converts ganciclovir (GCV) into a toxic product and allows selective elimination of TK+ cells in vitro and in vivo. It is …
Multiplexing RMCE: Versatile Extensions of the Flp ... - ScienceDirect
Sep 10, 2010 · Ganc enables the survival only of cells that have lost the HSV-tk function—ideally those that underwent RMCE (cf. Fig. 7).
Structures of replacement vectors for efficient gene targeting
When structures of the vector DNA fragments present in 20 G418-resistant and GANC-resistant non-targeted clones were examined by Southern blot analysis, the inefficiency of GANC …