Principles of genetic circuit design | Nature Methods
2014年4月29日 · This Review describes new tools that aid the construction of genetic circuits. Circuit dynamics can be influenced by the choice of regulators and changed with expression 'tuning knobs'.
Foundations for the design and implementation of synthetic …
2012年5月18日 · The emergence of a formalized design process for synthetic gene circuits represents one of the most important current developments in synthetic biology. In bottom-up assembly, engineers survey ...
Synthetic gene circuits for the detection, elimination and ... - Nature
2018年6月11日 · The circuit employed genetic building blocks of exclusively human origin to create a two-step sensory cascade that operates therapeutic transgene expression from synthetic STAT3-responsive promoters.
Genetic circuit engineering - Latest research and news | Nature
2024年10月17日 · Genetic circuit engineering is the synthesis of unnatural DNA segments encoding protein or RNA molecules that control each other’s levels.
Designing cell function: assembly of synthetic gene circuits for cell ...
2018年6月1日 · Recent advances in the development of genetic tools and the assembly of progressively more sophisticated gene circuits have made ‘designer cells’ a reality, with applications ranging from ...
Genetic circuit characterization by inferring RNA polymerase …
2020年10月5日 · Here, we take snapshots of a large genetic circuit in different states: RNA-seq is used to visualize circuit function as a changing pattern of RNA polymerase (RNAP) flux along the DNA.
Large-scale design of robust genetic circuits with multiple ... - Nature
2017年3月27日 · We present a robust, general, scalable system, called 'Boolean logic and arithmetic through DNA excision' (BLADE), to engineer genetic circuits with multiple inputs and outputs in mammalian cells ...
High-resolution and programmable RNA-IN and RNA-OUT …
2024年10月10日 · This study presents an RNA-IN and RNA-OUT genetic circuit capable dynamically sensing and manipulating any RNA target in a programmable manner.
Uncovering the genetic circuits that drive diseases | Nature ...
2023年7月25日 · A hierarchical Bayesian method identifies cell-type specific changes in gene regulatory circuits in disease by integrating single-cell and three-dimensional measurements of the genome.
Genetic circuit design automation with Cello 2.0 - Nature
2022年2月23日 · This protocol describes how to design genetic circuits using the web implementation of the Cello 2.0 software, which allows users to construct a DNA sequence for a genetic circuit composed of ...