One-step embedding method for maintaining orientation of …
In this study, we present a method of maintaining tissue fragment order and orientation using a thin film of ultra-high-strength agar and evaluate its usefulness during tissue sectioning. Cassettes were prepared, each containing three pieces of porcine liver, and compared embedding time with and without agar thin films (ATFs).
Handling of specimens and orientation of biopsies - Abdominal Key
Dec 15, 2022 · All tissues should be orientated and embedded in a way that will facilitate optimal microtomy. GIT biopsies should be embedded on edge so that the mucosal surface is on one side (Figure 13.2). Figure 13.1 (a) GIT biopsies embedded in multicassettes (e.g., ABC). The macroscopic description should indicate which sample corresponds to which suffix.
The effective use of gastrointestinal histopathology: guidance for ...
Biopsy and histological assessment provide a critical adjunct to endoscopic assessment of the gastrointestinal tract and, in diseases such as cancer, coeliac disease and chronic inflammatory bowel disease, pathological diagnosis remains the gold standard.
Histotechniques - University of Utah
Tissues from the body taken for diagnosis of disease processes must be processed in the histology laboratory to produce microscopic slides that are viewed under the microscope by pathologists. The techniques for processing the tissues, whether biopsies, larger specimens removed at surgery, or tissues from autopsy, are described below.
GI Biopsies & EMRs | Gross Pathology Manual - University of …
Nov 15, 2022 · In the clinical history, record the pertinent endoscopic findings found in the accompanying endoscopy report. Include the clinical history and indication for the procedure, endoscopic findings (gross appearance/lesions), and a summary of the impression.
Endoscopy, Tissue Processing, Stains, and Special Tests
Jun 25, 2019 · Endoscopic ultrasound tissue acquisition via fine-needle aspiration (FNA) cytology or fine-needle biopsy (FNB) using specialized core needles designed to obtain histological specimens has become a central technique in the assessment of GI and non-GI malignancies.
The effective use of gastrointestinal histopathology: guidance for ...
The effective use of gastrointestinal histopathology: guidance for endoscopic biopsy in the gastrointestinal tract. This is the first of three articles, published in Frontline Gastroenterology, that provides practical guidance of what to, and what not to, biopsy in the gastrointestinal (GI) tract.
Guidance on the effective use of upper gastrointestinal …
Jan 17, 2014 · Mesenchymal masses, such as gastrointestinal stromal tumour and leiomyoma, are usually submucosal or intramural, and will not be sampled by endoscopic biopsy, unless ulcerating the mucosal surface. If a submucosal (or deeper) lesion is suspected, this should be indicated clearly on the histology request form, so the pathologist is alerted to ...
The specimen handling of GI mucosal biopsy: a simple and
Objective: Histologic diagnostic foci on GI mucosal biopsy may be patchy. Therefore, slides with good orientation of mucosal tissue in a perpendicular plane and demonstrating an entire layer of mucosa will increase the diagnostic yield.
Low-cost workflow improvement reduces gastrointestinal block …
Methods: We implemented three specific changes: (1) up to 10 GI biopsy specimens could be placed in each cassette, (2) histotechnologists would no longer orient GI biopsy specimens, and (3) embedding would be in a straight line rather than diagonal. We evaluated the effects of these changes on total block numbers, quality of slides, and ...