DEAD-box RNA helicase subunits of the Drosha complex are
2007年4月15日 · Small interfering RNA (siRNA) oligonucleotides of p68, p72 and Drosha were used for knockdown of endogenous protein expression using siGENOME (Dharmacon, Lafayette, CO). Four kinds of independent...
Structure of Human DROSHA - ScienceDirect
2016年1月14日 · MicroRNA maturation is initiated by RNase III DROSHA that cleaves the stem loop of primary microRNA. DROSHA functions together with its cofactor DGCR8 in a heterotrimeric complex known as Microprocessor. Here, we report the X-ray structure of DROSHA in complex with the C-terminal helix of DGCR8.
Emerging roles of DROSHA beyond primary microRNA processing
DROSHA is the catalytic subunit of the Microprocessor complex, which initiates microRNA (miRNA) maturation in the nucleus by recognizing and cleaving hairpin precursors embedded in primary transcripts. However, accumulating evidence suggests that not all hairpin substrates of DROSHA are associated with the generation of functional small RNAs.
Drosha - Wikipedia
Drosha is a Class 2 ribonuclease III enzyme [5] that in humans is encoded by the DROSHA (formerly RNASEN) gene. [6] [7] [8] It is the primary nuclease that executes the initiation step of miRNA processing in the nucleus. It works closely with DGCR8 and in correlation with Dicer. It has been found significant in clinical knowledge for cancer ...
Drosha drives the formation of DNA:RNA hybrids around DNA
2018年2月7日 · Drosha is required within minutes of break induction, suggesting a central and early role for RNA processing in DNA repair. Sequencing of DNA:RNA hybrids reveals RNA invasion around DNA break...
生命学院王宏伟和生物物理研究所许瑞明课题组合作在《分子细胞 …
该结构证实了Drosha在切割位点界定中的决定性作用，并首次明确了PAZ、MB helix、以及dsRBD结构域分别对pri-miRNA的相应区域进行识别，协同完成切割位点的定位。
Structure of Human DROSHA - Cell Press
2016年1月14日 · The heretofore-elusive structure of DROSHA provides insights into how nascent microRNAs are recognized and cleaved in their initial processing step and reveals an unexpected similarity between DROSHA and DICER, the enzyme …
RNase III nucleases from diverse kingdoms serve as antiviral ... - Nature
2017年6月28日 · Here we demonstrate that Drosha and related RNase III ribonucleases from all three domains of life also elicit a unique RNA-targeting antiviral activity.
2016年1月14日 · DROSHA functions together with its cofactor DGCR8 in a heterotrimeric complex known as Microprocessor. Here, we report the X-ray struc-ture of DROSHA in complex with the C-terminal helix of DGCR8. We find that DROSHA contains two DGCR8-binding sites, one on each RNase III domain (RIIID), which mediate the assembly of Micropro-cessor.
human genome encodes only two RNase III proteins: Drosha and Dicer. Whereas Dicer homologs are found in a broad range of eukaryotic organisms, Drosha homologs are present only in animals but not in yeast or plants. Drosha was initially identified in Drosophila as an open reading frame adja-cent to the RNase H gene (3). The human homolog was ...