Introduction to Metabolic flux - University of Utah
Metabolism refers to a network of chemical reactions that occur within our cells and bodies to sustain life. Metabolic flux is a key concept while learning about homeostasis and regulation of these reaction pathways.
Metabolic Flux - an overview | ScienceDirect Topics
Metabolic flux is the level of metabolite flow in a metabolic pathway or the in vivo rate of an enzyme reaction, and it is defined as the number of converted molecules per unit time per cell (mol h − 1 cell − 1) (Stephanopoulos et al., 1998).
Metabolic flux analysis: a comprehensive review on sample …
Metabolic flux analysis (MFA) quantitatively describes cellular fluxes to understand metabolic phenotypes and functional behaviour after environmental and/or genetic perturbations.
A guide to metabolic flux analysis in metabolic engineering: …
2021年1月1日 · In this review, we describe the basic principles of metabolic flux analysis, discuss current best practices in flux quantification, highlight potential pitfalls and alternative approaches in the application of these tools, and give a broad overview of pragmatic applications of flux analysis in metabolic engineering practice.
High-resolution 13 C metabolic flux analysis - Nature
2019年8月30日 · Precise quantification of metabolic pathway fluxes in biological systems is of major importance in guiding efforts in metabolic engineering, biotechnology, microbiology, human health, and...
Quantitative flux analysis in mammals | Nature Metabolism
2021年7月1日 · Here, we review techniques for measuring metabolic fluxes in intact mammals and discuss how to analyse and interpret the results.
Tracing metabolic flux in vivo: basic model structures of tracer ...
2022年9月8日 · In this review, we discuss (1) the historical background of the use of stable isotope tracer methodology in metabolic research; (2) the importance of obtaining kinetic information for a better...
Defining the Metabolome: Size, Flux, and Regulation - Cell Press
2015年5月21日 · Metabolic fluxes are the in vivo velocities of metabolic reactions. These include the rate of transformation of intermediates by enzymes and of the transport of metabolites between compartments. In single cells or multicellular organisms, metabolic fluxes are an emerging property, as they depend on the systemic organization and interplay of ...
Integrative metabolic flux analysis reveals an indispensable …
Metabolic flux analysis (MFA) finds the set of fluxes that best recapitulate the observed growth rate, transport fluxes, and isotope labeling patterns across metabolites resulting from stable isotope tracers .
Metabolic Flux Lesson - University of Utah
Identify ways to measure metabolic flux. This lesson supports education of a 'Threshold Concept' in Biochemistry - Biochemical pathway dynamics and regulation identified by the American Society for Biochemistry and Molecular Biology (ASBMB).
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