(PDF) Simian Y chromosomes: species-specific ... - ResearchGate
1998年3月1日 · To map the pseudoautosomal boundary on the Y (PABY), probe 37E8 (containing sequences of XG and SRY ), and/or probe pHu14 (containing SRY sequences) were applied.
Nucleosome Positioning Signals in the DNA Sequence of the …
2003年1月31日 · Plasmids pHU41 (a) and pHU14 (b) mapped the upstream and downstream boundaries, respectively, of nucleosomes positioned by the R5–R7 repeats (represented by arrows that indicate the direction of cloning). Grey arrowheads indicate the boundaries of the methylation-inhibited R7 nucleosome that is described further in Figure 5.
We have investigated, by fluorescence in situ hybridization (FISH), the cytogenetic evolution of the Y chromosome in primates using 17 yeast artificial chromo- somes, representative of the Y-specific euchromatic re- gion of the human chromosome Y.
In Vitro Assembly of Human H/ACA Small Nucleolar RNPs Reveals …
Plasmid pHU14.5 encoding human U14 contains intron 5 of the HSC70 gene cloned between the XbaI and EcoRI sites of pBluescript KS(−). A DNA fragment encoding human RNase P RNA (H1) was cloned between the Eco RI and Xba I sites of pBluescript KS(−).
(PDF) Evolution of chromosome Y in primates - ResearchGate
1998年10月1日 · We have investigated, by fluorescence in situ hybridization (FISH), the cytogenetic evolution of the Y chromosome in primates using 17 yeast artificial chromosomes, representative of the Y-specific...
Male Infertility Caused by a - Oxford Academic
2000年11月1日 · Plasmid probes 7C46A for the DAZ gene (probe D, Fig. 1A) and pHu14 for the SRY gene were labeled with biotinylated deoxy-ATP or digoxigenated deoxyuridine 5-triphosphate (Roche, Molecular Biochemicals, Mannheim, Germany) by nick translation (Life Technologies, Inc.) and hybridized to chromosomes, nuclei, and DNA fibers, as previously described .
pBE14 - Addgene
Plasmid pBE14 from Dr. Huimin Zhao's lab contains the insert Cre and is published in Nat Commun. 2021 Feb 19;12 (1):1171. doi: 10.1038/s41467-021-21275-4. This plasmid is available through Addgene.
pHUE411 - Addgene
Plasmid pHUE411 from Dr. Qi-Jun Chen's lab contains the inserts gRNA scaffold and zCas9 and is published in BMC Plant Biol. 2014 Nov 29;14(1):327. This plasmid is available through Addgene.
Incomplete masculinisation of XX subjects carrying the SRY …
1999年7月1日 · For localisation of the Yp fragment, the pHu14 probe containing the human SRY gene was used. Discrimination between the active and inactive X chromosome (Xa and Xi, respectively) was done by...
Plasmid evolution: Current Biology - Cell Press
2020年10月5日 · Plasmids are genetic elements that colonize and replicate in prokaryotic cells (Box 1). They are considered a major driving force of prokaryote evolution, as they can migrate between populations, making them potent agents of lateral DNA transfer and microbial warfare.