Ribo-seq：检测正在翻译的mRNA信息，连接转录组学与蛋白质组 …
网页核糖体印迹测序 (Ribosome profiling, Ribo-seq)，能够详细检测体内的翻译状态。. Ribo-seq的技术核心是识别与核糖体结合的mRNA以及正在被翻译的约30个核苷酸。. 对核糖体结合的mRNA片段进行测序，能够精确记录核糖体在翻译过程中的位置。. 将转录组 …
Ribosome profiling - Wikipedia
网页Ribosome profiling, or Ribo-Seq (also named ribosome footprinting), is an adaptation of a technique developed by Joan Steitz and Marilyn Kozak almost 50 years ago that Nicholas Ingolia and Jonathan Weissman adapted to work with next generation sequencing that uses specialized messenger RNA (mRNA) sequencing to …
干货分享|Ribo-seq：组学串联的桥梁 - 知乎 - 知乎专栏
网页2023年10月8日 · 翻译组+转录组——明确定位 组学搭配更出效果. 翻译组（Ribo-seq）加上精准定量的转录组（UMI转录组），不仅可以揭示 mRNA 翻译效率，还可以在翻译层面解释翻译组与蛋白组数据的衔接机制，如翻译效率下降而转录本水平不变导致的蛋白水平的变化。. …
Ribo-seq建库测序原理和应用 - 简书
网页2023年7月20日 · 翻译组测序（Ribo-seq）是指 对与核糖体结合的正在翻译的RNA片段进行测序 ，来准确获取样本中 所有可翻译分子 （包括mRNA和其他潜在可翻译RNA分子如lncRNA, circRNA等）的信息与精确定量，是连接转录组与蛋白质组之间的桥梁。. Ribo-seq是最常用的一种翻译组测序 ...
Ribo-Seq
网页RiboSeq.Org hosts a collection of tools for the processing, analysis and visualization of ribosome profiling data. Explore over 13,000 Ribo-Seq samples in your browser or download processed files for further analysis offline.
Ribosome profiling reveals the what, when, where and how of …
网页2015年10月14日 · Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA fragments, is a powerful tool for globally monitoring translation in vivo.
Tracing Translational Footprint by Ribo-Seq: Principle, Workflow, …
网页2022年9月23日 · Ribo-seq (or ribosome profiling) is a deep-sequencing-based high-throughput method that measures the abundance of transcripts bounded by ribosomes. Given that most protein assays are low-throughput, Ribo-seq provides a unique technology that systematically measures translated transcripts.
Single-cell Ribo-seq reveals cell cycle-dependent ... - Nature
网页2021年9月8日 · Single-cell ribosome sequencing (scRibo-seq) combines nuclease footprinting with small-RNA library construction and a size enrichment to measure translation dynamics in single cells...
Principles, challenges, and advances in ribosome profiling: from …
网页2023年12月1日 · Ribosome profiling, also known as Ribo-seq, is a technology that provides a snapshot of global translation in cells by sequencing the RNA fragments protected by translating ribosomes (Ingolia et al. 2009; Brar and Weissman 2015).
Uncovering the small proteome of Methanosarcina mazei using Ribo-seq …
网页2 天之前 · Set up of Ribo-seq optimized for M. mazei. To generate a translatome map that provides a comprehensive depiction of translated annotated sORFs and to discover unannotated sORFs in M. mazei Gö1 ...