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  1. Springer
    https://link.springer.com/article/10.1007/s00216-011-4764-2

    Mitochondrial ROS production under cellular stress: …

    • The cultivation and processing of 32D cells for all experiments was carried out as previously described . HL-1 cells with cardiac phenotype were grown in fibronectin–gelatine-coated flasks containing Claycom… 展开

    Confocal Microscopy

    Cells were placed in Lab-Tek chambered cover glass (Nalge Nunc, Rochester, NY, USA) filled with normal medium (chamber volume 0.3 ml, 10 × 103–20 × 103 cells per chamber). For c… 展开

    Springer
    Determination of Ros by ESR

    ESR has clear ROS specificity. Cellular or tissue contents of superoxide radicals and peroxynitrite are detected by ESR, but not hydrogen peroxide. HL-1 cardiomyocytes, 32D cells, or isol… 展开

    Springer
    Cellular Stress Conditions

    Growth factor abrogation in 32D cells was achieved by extensively washing cells in standard tissue culture medium and reseeding the cells in IL-3-free medium for 8-16 h as previo… 展开

    Springer
    Materials

    All chemicals, including the mitochondria-specific fluorescent dye, were obtained from Sigma (St. Louis, MO, USA) except for MitoTracker® red CM-H2XRos and MitoTracker® de… 展开

    Springer
    What is a common Ros?
    Common ROS consist of superoxide (O2−), hydrogen peroxide (H 2 O 2), peroxynitrite (ONOO−), reactive aldehydes, nitric oxide (NO) and other hydroxyl radicals [6, 7]. In this process, molecular oxygen is sequentially reduced in mitochondria by the addition of four electrons to synthesize water.
    sciencedirect.com
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    目录
    Confocal Microscopy
    Determination of Ros by ESR
    Cellular Stress Conditions
    Materials
    What is a common Ros?
     
  1. nih.gov
    https://pmc.ncbi.nlm.nih.gov/articles/PMC8275304

    Intracellular and Mitochondrial Reactive Oxygen Species …

  2. Nature
    https://www.nature.com/articles/s41586-01…

    RGF1 controls root meristem size through ROS signalling

    2019年12月4日 · ROS are an intrinsic signal for establishing the size of the meristematic zone: superoxide (O 2 −) accumulates primarily in the meristematic zone, hydrogen peroxide (H 2 O 2) accumulates mainly...

  3. Ros Confocal 的研究
    ScienceDirect
    Fluorescent in vivo imaging of reactive oxygen species and redox potential in plants
    Reactive oxygen species (ROS) are by-products of aerobic metabolism, and excessive production can result in oxidative stress and cell damage. In addition, ROS function as cellular messengers, working as redox regulators in a multitude of biological processes. Understanding ROS signalling and stress ...
    已发布: 2018/07/01
    Keywords: 2-OH-E+ 2-…
    页数: 19
    ScienceDirect
    Research paper On-chip real-time detection and quantification of reactive oxygen species in MCF-7 cells through an in-house built fluorescence microscope
    Reactive oxygen species (ROS) are formed by several different reactions and play a major role in cell cycling and as a messenger in normal cell transduction and have diverse biological effects when high in concentration. ROS may trigger DNA damage, cell mutation, inflammation, cell proliferation, an...
    已发布: 2020/09/15
    Cited By: 0
    Keywords: Fluorescen…
    PubMed
    Mitochondrial ROS production under cellular stress: comparison of different detection methods
    Reactive oxygen species (ROS) are involved in the regulation of many physiological processes. However, overproduction of ROS under various cellular stresses results in cell death and organ injury and thus contributes to a broad spectrum of diseases and pathological conditions. The existence of diffe...
    作者: Andrey V Kuznet…
    已发布: 2011/6
    Keywords: National In…
    ScienceDirect
    Research Paper Non-invasive real-time imaging of reactive oxygen species (ROS) using auto-fluorescence multispectral imaging technique: A novel tool for redox biology
    Detecting reactive oxygen species (ROS) that play a critical role as redox modulators and signalling molecules in biological systems currently requires invasive methods such as ROS -specific indicators for imaging and quantification. We developed a non-invasive, real-time, label-free imaging techniq...
    已发布: 2020/07/01
    Cited By: 0
    DOI: 10.1016/j.redox.2…
    PubMed
    Monitoring reactive oxygen species formation and localisation in living cells by use of the fluorescent probe CM-H(2)DCFDA and confocal laser microscopy
    Reactive oxygen species (ROS) develop as a consequence of wounding, light stress and chemical imbalances but act also as signals in living cells. The integrity of cells is seriously endangered, if ROS cannot be controlled by scavenging molecules and other repair mechanisms of the cell. For studying ...
    作者: Kim Anker Kristia…
    已发布: 2009/8
    Keywords: National In…
    PubMed
    Imaging ROS signaling in cells and animals
    Reactive oxygen species (ROS) act as essential cellular messengers, redox regulators, and, when in excess, oxidative stressors that are widely implicated in pathologies of cancer and cardiovascular and neurodegenerative diseases. Understanding such complexity of the ROS signaling is critically hinge...
    作者: Xianhua Wang
    已发布: 2013/8
    Keywords: National In…
  4. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S104620232200175X

    Detection and quantification of nanoparticle-induced intracellular …

  5. nih.gov
    https://pmc.ncbi.nlm.nih.gov/articles/PMC8520137

    Live Cell Imaging of Mitochondrial Redox State in Mammalian …

  6. Nature
    https://www.nature.com/articles/s42255-022-00591-z

    Guidelines for measuring reactive oxygen species and oxidative …

  7. 其他用户还问了以下问题
    Can confocal microscopy capture Ros dynamics in vivo?
    Imaging ROS dynamics in vitro and in vivo. By combining transgenic animal models with confocal and multiphoton microscopy, images of high spatiotemporal resolution can now be acquired from isolated mitochondria, cultured cells, intact tissues or organs, and even in living animals

    Imaging ROS signaling in cells and animals | Journal of ... - Springer

    link.springer.com
    Can extracellular Ros be detected by confocal fluorescent microscopy?
    The latter is of particular interest because extracellular ROS cannot always be properly detected by confocal fluorescent microscopy and in this case ESR analysis would serve as a necessary complementary approach.

    Mitochondrial ROS production under cellular stress: comparison of

    link.springer.com
    What is a common Ros?
    Common ROS consist of superoxide (O2−), hydrogen peroxide (H 2 O 2), peroxynitrite (ONOO−), reactive aldehydes, nitric oxide (NO) and other hydroxyl radicals [6, 7]. In this process, molecular oxygen is sequentially reduced in mitochondria by the addition of four electrons to synthesize water.

    On-chip real-time detection and quantification of reactive oxygen speci…

    sciencedirect.com
    How complex is ROS signaling?
    However, ROS signaling is notoriously complex. As a rule of thumb, the ROS effects are multiphasic and bidirectional, depending on the species of oxidants, their concentrations, history of exposure, and cellular context.

    Imaging ROS signaling in cells and animals | Journal of ... - Springer

    link.springer.com
    What is the role of ROS in cellular homeostasis?
    The modulation of intracellular reactive oxygen species (ROS) levels is crucial for cellular homeostasis and determination of cellular fate. A sublethal level of ROS sustains cell proliferation, differentiation and promotes tumor metastasis, while a drastic ROS burst directly induces apoptosis.

    Arsenene-mediated multiple independently targeted reactive oxygen

    nature.com
    Why is ROS imaging important?
    Innovative ROS imaging has led to important discoveries in ROS signaling—for example, mitochondrial superoxide flashes as elemental ROS signaling events and hydrogen peroxide transients for wound healing.

    Imaging ROS signaling in cells and animals | Journal of ... - Springer

    link.springer.com
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  8. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0891584918301692

    Fluorescent in vivo imaging of reactive oxygen species and redox ...

  9. Nature
    https://www.nature.com/articles/s41467-021-24961-5

    Arsenene-mediated multiple independently targeted reactive …

  10. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0167931720302203

    On-chip real-time detection and quantification of reactive oxygen ...

  11. Springer
    https://link.springer.com/article/10.1007/…

    Imaging ROS signaling in cells and animals | Journal of …

    2013年7月20日 · Reactive oxygen species (ROS) act as essential cellular messengers, redox regulators, and, when in excess, oxidative stressors that are widely implicated in pathologies of cancer and cardiovascular and …