Efficacy of red light for enhanced cell disruption and fluorescence ...
2020年9月4日 · In this study, red LED and urea used as light and nitrogen sources, respectively, for the cultivation of Spirulina to enhance the fluorescence property and purity of phycocyanin. Besides, there is a high concentration of phycocyanin leached out from red light (RL) grown cells than white light (WL) without cell disruption.
Chlorophyll-a measurements conducted using Spirulina …
Recently, Cadondon et al., [121] used a portable pulsed LED fluorescence LiDAR system to quantify chlorophyll 'a' concentration in Spirulina algae. They found a strong correlation between the...
Structural insights reveal the effective Spirulina platensis cell …
2020年3月1日 · Spirulina platensis cell wall dissociation mechanisms, via attacks by OH − or H +, were proposed. These treatments caused the cell walls to expose more surface spaces and release their contents into the supernatant, enhancing the algal cells’ interactions with agents from the extracting, cracking, and generating steps, and leading to an ...
Chlorophyll-a Pigment Measurement of Spirulina in Algal Growth ...
This is the first study that compared range resolved fluorescence peaks of Spirulina using a new developed lidar system with absorbance, fluorescence, and corrected chlorophyll-a measurements. Chlorophyll fluorescence parameters is the extracted lidar fluorescence intensity ratio at 680 nm to that of 700 nm (F680/F700) [ 86 ].
Exploring effective light spectral conversion techniques for …
2024年5月1日 · Researchers have employed fluorescent lamps as a lighting source in Spirulina-based pigment productions. For instance, Markou et al. (2021) cultivated A. platensis SAG 21.99 in Zarrouk’s medium as the control under the illumination of cool-white fluorescent tubes at 300 μmol m −2 s −1 during an investigation on the microalgal growth in ...
Comparison of Pressurized Water Extraction With Ultrasound …
1 天前 · The results showed that the sequential PWE of individual Spirulina samples was repeatable and the mild extraction provided the fluorescent pI profile similar to the commercially available C-phycocyanin standard. Collectively, the results obtained encourage the use of PWE in small-scale analytical applications for primary extraction of proteins ...
Photosynthetic characteristics of Spirulina platensis on solid …
Several aspects of photosynthesis in vivo (CO 2 and O 2 exchange, chlorophyll fluorescence and its quenching components) were measured in Spirulina platensis after filtering suspensions of this cyanobacteria through fibre glass filters. This simple procedure allowed the use of methods which previously could be applied only to leaves.
Photosynthetic characteristics of Spirulina platensis grown in ...
2007年4月4日 · Using chlorophyll a fluorescence, several researchers found a midday decrease in F v /F m (variable fluorescence over maximal fluorescence, or the maximum yield of primary photochemistry of dark adapted cells) which has been interpreted as a decrease in photosynthetic efficiency as a result of photo-inhibition (Vonshak and Torzillo 2004).
Far‐red dependent changes in the chemical composition of Spirulina …
2016年5月3日 · The fluorescence emission spectra of Spirulina platensis cells in the blue–green range during growth under (A) WL, (B) BRL, and (C) BRFRL. Fluorescence emission spectra can produce many relevant information regarding plant responses to changing growth conditions, for example, the intensity and/or spectrum of light, temperature, and nutrient ...
Two-Dimensional and Three-Dimensional Raman Spectroscopic
2022年11月12日 · Raman spectra exhibited an evident sign of fluorescence, which changed during cumulative laser illumination on the same spot. Six peaks were selected from the spectra for chemical mapping, which showed distinct cellular features, including the central and peripheral parts of the cytoplasm, cell wall, sheath, and medium residues.