Purification and analysis of bispecific tetrameric antibody complexes ...
网页This relatively simple cell fractionation technique illustrates the unique cross-linking properties of bispecific tetrameric antibody complexes. The procedure appears useful for further studies of hemopoietic cells and bone marrow transplantation.
Tetrameric Antibody Complexes and Affinity Tag Peptides for …
网页2023年5月27日 · Here, we present a novel strategy for the selective immobilization of single QD-peptide conjugates using a combination of tetrameric antibody complexes (TACs) and affinity tag peptides. A glass substrate is modified with an adsorbed layer of concanavalin A (ConA) that binds a subsequent layer of dextran that minimizes nonspecific binding.
Collection, Storage, and Preparation of Human Blood Cells
网页2016年5月1日 · There are modifications of this method to enrich unlabeled cells of interest by using Rosettesep Tetrameric antibody complexes (a cocktail of bi-specific antibodies to preferentially cross link undesired cells to RBCs) (Stem Cell Technologies) which remove unwanted cells by crosslinking and pelleting together with RBCs.
Cyclic tetramolecular complexes of monoclonal antibodies: a …
网页Tetrameric antibody complexes are more easily prepared than bispecific antibodies or bifunctional antibodies produced by transfection of myelomas with recombinant genes. They also have the advantage that the antigen-binding properties of the bivalent monoclonal antibodies are not compromised.
More tricks with tetramers: a practical guide to staining T cells with ...
网页2015年6月16日 · These techniques are: (i) using a bright fluorochrome, (ii) inclusion of a PKI during staining, (iii) staining with anti-coreceptor antibody after staining with pMHC multimer, (iv) use of higher-order multimers, and (v) …
Implementing a method for engineering multivalency to …
网页2021年5月18日 · The method yields tetrameric antibodies, termed quads, that retain efficient binding to the SARS-CoV-2 spike protein, show up to two orders of magnitude enhancement in neutralization of...
Cyclic tetramolecular complexes of monoclonal antibodies: a
网页Tetrameric antibody complexes are more easily prepared than bispecific antibodies or bifunctional antibodies produced by transfection of myelomas with recombinant genes. They also have the advantage that the antigen-binding properties of the bivalent monoclonal antibodies are not compromised.
Specific binding and release of cells from beads using cleavable ...
网页Tetrameric antibody complexes are a powerful new type of cross‐linking reagent that may have a wide spectrum of applications in biology and medicine and have the advantage that the antigen‐binding properties of the bivalent …
Use of tetrameric antibody complexes to stain cells for flow …
网页The ability of such cyclic immune complexes to stain T-cell subset antigens on human peripheral blood lymphocytes was demonstrated in single and double-staining experiments. The results demonstrate that tetrameric antibody complexes provide a simple and efficient alternative to covalently labeled antibodies for the flow cytofluorimetric ...
Use of tetrameric antibody complexes to stain cells for flow
网页Bifunctional tetrameric complexes of monoclonal antibodies were used to stain cells for flow cytometry. These complexes consist of two different mouse monoclonal IgG1 antibodies (one with specificity for a cell surface antigen, the other with specificity for a fluorochrome) cross-linked by two molec ….
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