Purification and analysis of bispecific tetrameric antibody complexes ...
This relatively simple cell fractionation technique illustrates the unique cross-linking properties of bispecific tetrameric antibody complexes. The procedure appears useful for further studies of hemopoietic cells and bone marrow transplantation.
Tetrameric Antibody Complexes and Affinity Tag Peptides for …
May 27, 2023 · Here, we present a novel strategy for the selective immobilization of single QD-peptide conjugates using a combination of tetrameric antibody complexes (TACs) and affinity tag peptides. A glass substrate is modified with an adsorbed layer of concanavalin A (ConA) that binds a subsequent layer of dextran that minimizes nonspecific binding.
A super-potent tetramerized ACE2 protein displays enhanced ...
May 19, 2021 · The ACE2 tetrameric protein complex promise to be important for development as decoy therapeutic proteins against COVID-19. In contrast to monoclonal antibodies, ACE2 decoy is unlikely to...
Use of tetrameric antibody complexes to stain cells for flow
Bifunctional tetrameric complexes of monoclonal antibodies were used to stain cells for flow cytometry. These complexes consist of two different mouse monoclonal IgG1 antibodies (one with specificity for a cell surface antigen, the other with specificity for a …
Pre-existing antibodies directed against a tetramerizing ... - Nature
Jan 27, 2022 · Antibodies can form immune complexes that promote adaptive immune responses against the complexed antigen by the interaction of the Fc domain of these antibodies with activating Fcγ receptors ...
Specific binding and release of cells from beads using cleavable ...
Tetrameric antibody complexes are a powerful new type of cross‐linking reagent that may have a wide spectrum of applications in biology and medicine and have the advantage that the antigen‐binding properties of the bivalent monoclonal antibodies are not compromised.
Cyclic tetramolecular complexes of monoclonal antibodies: A …
Tetrameric antibody complexes are more easily prepared than bispecific antibodies or bifunctional antibodies produced by transfection of myelomas with recombinant genes. They also have the advantage that the antigen‐binding properties of the bivalent monoclonal antibodies are …
Specific binding and release of cells from beads using cleavable ...
Jun 21, 1989 · In the first step cells are specifically cross-linked to hapten-modified glass beads using tetrameric monoclonal antibody complexes. In the second step bound cells are selectively eluted by reductive cleavage of the tetrameric antibody complexes.
Isolation of Subsets of Immune Cells - Springer Nature
Subsets of immune cells can be isolated before analysis by the enzyme-linked immunospot (ELISPOT) assay with various cell separation techniques. This chapter describes techniques to select desired cells or deplete unwanted cells by crosslinking cells. … more.
A soluble activator that favors the ex vivo expansion of CD8
Nov 19, 2020 · Human T cells are presently expanded in culture through the use of anti-CD3 and anti-CD28 mAbs immobilized on beads, expressed on cells, or assembled in the context of soluble antibody complexes. Here we report the design of a small, bispecific single-chain variable fragment construct agonizing both CD3 and CD28 pathways.