Ultrasound-responsive nanocarriers with siRNA and Fe3O4 …
2024年10月7日 · To finalize the binding of siRNA to FA-PFNB (FA@PFP@Fe 3 O 4 @LNBs), the positively charged FA-PFNB was merged with the siRNA solution in enzyme-free water and incubated at room temperature for 10 min. The resulting prepared samples were …
127aa encoded by circSpdyA promotes FA synthesis and NK cell
2024年10月14日 · Carnitine palmitoyltransferase I (CPT1) is the key rate-limiting enzyme of FAO. We next knocked down CPT1 with a specific siRNA and subjected the cells to an OCR assay.
Structural optimization of siRNA conjugates for albumin binding ...
2024年2月21日 · These results demonstrate that increasing the EG spacer length diminishes the propensity for siRNA-lipid micellization, an important consideration when employing long FA chains for albumin...
Phase separation of SGS3 drives siRNA body formation and …
2023年1月31日 · Tan et al. show that SGS3 undergoes liquid-liquid phase separation that drives RDR6, RNAs, RNA-binding proteins, and other siRNA processing components to form siRNA bodies in cytoplasm.
SiRNA Delivery with PEGylated Graphene Oxide Nanosheets for …
2017年2月27日 · The human normal liver cell line QSG7701 was treated with different FA/GO/siRNA nanoformulations to estimate their specificities and toxicities (Fig. S5). The treatment of FA/GO/siRNA nanoformulations led to no obvious growth inhibition of …
Folate Receptor-Mediated siRNA Delivery: Recent Developments …
Folic acid (FA), due to its small size, low immunogenicity, high in vivo stability, and high binding affinity toward FRs, has attracted much attention for targeted siRNA delivery. FRs are overexpressed in a large number of tumors, including ovarian, breast, …
Folate-displaying exosome mediated cytosolic delivery of siRNA …
The double stranded siRNA against survivin conjugated with FA (FA-Sur-A647) without exosome was used as the control. Corresponding characterizations of RNA or exosomes are provided in Fig. S1 , S2 .
Design and preparation of a new multi-targeted drug delivery …
2021年4月1日 · Besides, siRNA-FAM internalization was investigated by fluorescence microscopy. The results showed the blank nanoparticles were significantly less cytotoxic at various concentrations. Meanwhile, siRNA-FAM/PTX encapsulated nanoparticles exhibited significant anticancer activity against MCF-7 and BT-474 cell lines.
Folic acid (FA)-conjugated mesoporous silica ... - ScienceDirect
2020年5月1日 · At first, the Myr-loaded MSNs conjugated with MRP-1 siRNA and FA was prepared. The results, for the first time, suggested that FA-conjugated MSNs combined with Myr and MRP-1 nanoparticles markedly reduced the proliferation of lung cancer cells, and induced apoptosis in NSCLC cells.
Self-Assembly of a Multifunction DNA Tetrahedron for Effective …
2022年7月7日 · Schematic representation of the antitumor therapy of TDN-FA/PL1/Pcsk9 siRNA. TDN-FA/PL1/Pcsk9 siRNA dissociated and released the Pcsk9 siRNA in tumor cells by FA/FAR recognition, thereby reducing PCSK9-mediated MHC I …